Detailed information of TA system TA193483

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 55162..55401
• Replicon: plasmid unnamed
• Accession: NZ_CP070171
• Organism: Escherichia coli strain FDAARGOS_1284

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: I6K14_RS23900
• Protein ID: WP_023144756.1
• Coordinates: 55162..55296 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	55341..55401 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K14_RS23865 (50958)	50958..51362	+	405	WP_000839179.1	transposase	-
I6K14_RS23870 (51359)	51359..51706	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
I6K14_RS23875 (51755)	51755..53294	+	1540	Protein_67	IS66-like element ISEc22 family transposase	-
I6K14_RS23880 (53450)	53450..54307	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
I6K14_RS23885 (54300)	54300..54374	-	75	WP_031943482.1	RepA leader peptide Tap	-
I6K14_RS23895 (54611)	54611..54865	-	255	WP_000083850.1	replication regulatory protein RepA	-
I6K14_RS23900 (55162)	55162..55296	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (55341)	55341..55401	+	61	NuclAT_2	-	Antitoxin
- (55341)	55341..55401	+	61	NuclAT_2	-	Antitoxin
- (55341)	55341..55401	+	61	NuclAT_2	-	Antitoxin
- (55341)	55341..55401	+	61	NuclAT_2	-	Antitoxin
I6K14_RS23905 (55368)	55368..55654	-	287	Protein_72	DUF2726 domain-containing protein	-
I6K14_RS23910 (56167)	56167..56379	-	213	WP_013023861.1	hypothetical protein	-
I6K14_RS23915 (56510)	56510..57070	-	561	WP_000139341.1	fertility inhibition protein FinO	-
I6K14_RS23920 (57125)	57125..57871	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catA1 / dfrA8 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	-	1..118581	118581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T193483 WP_023144756.1 NZ_CP070171:c55296-55162 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T193483 NZ_CP093011:c1722237-1721860 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT193483 NZ_CP070171:55341-55401 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin