Detailed information of TA system TA193481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4632180..4632782
• Replicon: chromosome
• Accession: NZ_CP070170
• Organism: Escherichia coli strain FDAARGOS_1284

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K14_RS22045
• Protein ID: WP_000897302.1
• Coordinates: 4632471..4632782 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K14_RS22040
• Protein ID: WP_000356397.1
• Coordinates: 4632180..4632470 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K14_RS22015 (4628245)	4628245..4629147	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K14_RS22020 (4629144)	4629144..4629779	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K14_RS22025 (4629776)	4629776..4630705	+	930	WP_087598274.1	formate dehydrogenase accessory protein FdhE	-
I6K14_RS22030 (4630929)	4630929..4631147	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K14_RS22035 (4631543)	4631543..4631821	-	279	WP_001296612.1	hypothetical protein	-
I6K14_RS22040 (4632180)	4632180..4632470	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K14_RS22045 (4632471)	4632471..4632782	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K14_RS22050 (4633011)	4633011..4633919	+	909	WP_204653509.1	alpha/beta hydrolase	-
I6K14_RS22055 (4633983)	4633983..4634924	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K14_RS22060 (4634969)	4634969..4635406	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K14_RS22065 (4635403)	4635403..4636275	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K14_RS22070 (4636269)	4636269..4636868	-	600	WP_001298594.1	glucose-1-phosphatase	-
I6K14_RS22075 (4636967)	4636967..4637752	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T193481 WP_000897302.1 NZ_CP070170:c4632782-4632471 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193481 NZ_CP093011:c1502167-1501703 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT193481 WP_000356397.1 NZ_CP070170:c4632470-4632180 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193481 NZ_CP093011:c1502502-1502167 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: S1P416