Detailed information of TA system TA193478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3765535..3766229
• Replicon: chromosome
• Accession: NZ_CP070170
• Organism: Escherichia coli strain FDAARGOS_1284

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K14_RS18035
• Protein ID: WP_001263500.1
• Coordinates: 3765535..3765933 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K14_RS18040
• Protein ID: WP_000554758.1
• Coordinates: 3765936..3766229 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K14_RS18010 (3760900)	3760900..3761358	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
I6K14_RS18015 (3761619)	3761619..3763076	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K14_RS18020 (3763133)	3763133..3763747	-	615	WP_000602123.1	peptide chain release factor H	-
I6K14_RS18025 (3763744)	3763744..3764883	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K14_RS18030 (3765073)	3765073..3765525	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K14_RS18035 (3765535)	3765535..3765933	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K14_RS18040 (3765936)	3765936..3766229	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K14_RS18045 (3766281)	3766281..3767336	-	1056	WP_001226168.1	DNA polymerase IV	-
I6K14_RS18050 (3767407)	3767407..3768192	-	786	WP_061336152.1	putative lateral flagellar export/assembly protein LafU	-
I6K14_RS18055 (3768164)	3768164..3769876	+	1713	Protein_3583	flagellar biosynthesis protein FlhA	-
I6K14_RS18060 (3769955)	3769955..3770299	+	345	WP_124072110.1	hypothetical protein	-
I6K14_RS18065 (3770294)	3770294..3770791	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T193478 WP_001263500.1 NZ_CP070170:c3765933-3765535 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193478 NZ_CP093011:c1109004-1108702 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT193478 WP_000554758.1 NZ_CP070170:c3766229-3765936 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193478 NZ_CP093011:c1108666-1108325 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3