Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2441765..2442327 Replicon chromosome
Accession NZ_CP070170
Organism Escherichia coli strain FDAARGOS_1284

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K14_RS11490 Protein ID WP_000605675.1
Coordinates 2441765..2442043 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K14_RS11495 Protein ID WP_000781370.1
Coordinates 2442043..2442327 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K14_RS11465 (2437351) 2437351..2437782 - 432 WP_000152305.1 peroxiredoxin OsmC -
I6K14_RS11470 (2438128) 2438128..2438343 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K14_RS11475 (2438445) 2438445..2438582 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K14_RS11480 (2438738) 2438738..2440435 + 1698 WP_000433462.1 malate dehydrogenase -
I6K14_RS11485 (2440569) 2440569..2441579 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K14_RS11490 (2441765) 2441765..2442043 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K14_RS11495 (2442043) 2442043..2442327 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K14_RS11500 (2442556) 2442556..2443209 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
I6K14_RS11505 (2443202) 2443202..2444086 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
I6K14_RS11510 (2444099) 2444099..2447146 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T193475 WP_000605675.1 NZ_CP070170:2441765-2442043 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T193475 NZ_CP093011:c452704-452426 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT193475 WP_000781370.1 NZ_CP070170:2442043-2442327 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT193475 NZ_CP093011:c452897-452706 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References