Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1823611..1824113 Replicon chromosome
Accession NZ_CP070170
Organism Escherichia coli strain FDAARGOS_1284

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6K14_RS08570 Protein ID WP_000767822.1
Coordinates 1823859..1824113 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K14_RS08565 Protein ID WP_001259255.1
Coordinates 1823611..1823862 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K14_RS08540 (1818789) 1818789..1819856 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6K14_RS08545 (1819856) 1819856..1820926 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
I6K14_RS08550 (1820923) 1820923..1822227 - 1305 WP_001297912.1 histidinol dehydrogenase -
I6K14_RS08555 (1822233) 1822233..1823132 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K14_RS08560 (1823278) 1823278..1823328 - 51 WP_001364200.1 his operon leader peptide -
I6K14_RS08565 (1823611) 1823611..1823862 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K14_RS08570 (1823859) 1823859..1824113 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K14_RS08575 (1824196) 1824196..1825020 + 825 WP_000754733.1 SDR family oxidoreductase -
I6K14_RS08580 (1825066) 1825066..1825995 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
I6K14_RS08585 (1826210) 1826210..1826272 + 63 WP_010723108.1 membrane protein YoeI -
I6K14_RS08590 (1826262) 1826262..1827620 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T193464 WP_000767822.1 NZ_CP070170:1823859-1824113 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T193464 NZ_CP092988:c43142-42819 [Leptospira interrogans]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGAGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCAT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTTGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT193464 WP_001259255.1 NZ_CP070170:1823611-1823862 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT193464 NZ_CP092988:c43384-43136 [Leptospira interrogans]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References