Detailed information of TA system TA193441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4804797..4805399
• Replicon: chromosome
• Accession: NZ_CP070162
• Organism: Escherichia coli strain FDAARGOS_1283

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K13_RS23345
• Protein ID: WP_000897302.1
• Coordinates: 4804797..4805108 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K13_RS23350
• Protein ID: WP_000356397.1
• Coordinates: 4805109..4805399 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K13_RS23315 (4799827)	4799827..4800612	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
I6K13_RS23320 (4800711)	4800711..4801310	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6K13_RS23325 (4801304)	4801304..4802176	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K13_RS23330 (4802173)	4802173..4802610	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K13_RS23335 (4802655)	4802655..4803596	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K13_RS23340 (4803660)	4803660..4804568	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6K13_RS23345 (4804797)	4804797..4805108	+	312	WP_000897302.1	hypothetical protein	Toxin
I6K13_RS23350 (4805109)	4805109..4805399	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6K13_RS23355 (4805758)	4805758..4806036	+	279	WP_001296612.1	hypothetical protein	-
I6K13_RS23360 (4806432)	4806432..4806650	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K13_RS23365 (4806874)	4806874..4807803	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K13_RS23370 (4807800)	4807800..4808435	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K13_RS23375 (4808432)	4808432..4809334	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T193441 WP_000897302.1 NZ_CP070162:4804797-4805108 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193441 NZ_CP092973:c1872556-1872179 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT193441 WP_000356397.1 NZ_CP070162:4805109-4805399 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193441 NZ_CP092973:c1873014-1872646 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: S1P416