Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3377213..3377940 Replicon chromosome
Accession NZ_CP070162
Organism Escherichia coli strain FDAARGOS_1283

Toxin (Protein)


Gene name higB Uniprot ID A0A8E0MHE9
Locus tag I6K13_RS16530 Protein ID WP_021524447.1
Coordinates 3377629..3377940 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6K13_RS16525 Protein ID WP_000126304.1
Coordinates 3377213..3377632 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K13_RS16510 (3373376) 3373376..3375628 - 2253 WP_021524446.1 carbamoyltransferase HypF -
I6K13_RS16515 (3375781) 3375781..3376308 - 528 WP_001078777.1 electron transport protein HydN -
I6K13_RS16520 (3376693) 3376693..3377121 + 429 WP_000536064.1 hypothetical protein -
I6K13_RS16525 (3377213) 3377213..3377632 - 420 WP_000126304.1 helix-turn-helix domain-containing protein Antitoxin
I6K13_RS16530 (3377629) 3377629..3377940 - 312 WP_021524447.1 type II toxin-antitoxin system HigB family toxin Toxin
I6K13_RS16535 (3378103) 3378103..3378858 - 756 WP_001314074.1 hypothetical protein -
I6K13_RS16540 (3378901) 3378901..3379371 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
I6K13_RS16545 (3379364) 3379364..3379774 - 411 WP_001291913.1 formate hydrogenlyase assembly protein HycH -
I6K13_RS16550 (3379771) 3379771..3380538 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I6K13_RS16555 (3380538) 3380538..3381080 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
I6K13_RS16560 (3381090) 3381090..3382799 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12428.24 Da        Isoelectric Point: 9.7074

>T193437 WP_021524447.1 NZ_CP070162:c3377940-3377629 [Escherichia coli]
MHIISKAPFEECARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNKLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T193437 NZ_CP092973:930314-930721 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15474.50 Da        Isoelectric Point: 4.4596

>AT193437 WP_000126304.1 NZ_CP070162:c3377632-3377213 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT193437 NZ_CP092973:930067-930333 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References