Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 976622..977301 Replicon chromosome
Accession NZ_CP070162
Organism Escherichia coli strain FDAARGOS_1283

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag I6K13_RS04560 Protein ID WP_000057524.1
Coordinates 976622..976924 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K13_RS04565 Protein ID WP_000806442.1
Coordinates 976960..977301 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K13_RS04535 (971998) 971998..973650 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
I6K13_RS04540 (973688) 973688..974191 - 504 WP_000667000.1 hypothetical protein -
I6K13_RS04545 (974188) 974188..974988 - 801 WP_113263503.1 hypothetical protein -
I6K13_RS04550 (975012) 975012..975491 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K13_RS04555 (975695) 975695..976489 - 795 WP_000365177.1 TraB/GumN family protein -
I6K13_RS04560 (976622) 976622..976924 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K13_RS04565 (976960) 976960..977301 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K13_RS04570 (977359) 977359..979863 - 2505 WP_021513535.1 copper-exporting P-type ATPase CopA -
I6K13_RS04575 (980125) 980125..981057 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T193420 WP_000057524.1 NZ_CP070162:976622-976924 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T193420 NZ_CP092968:3822406-3822852 [Methylocystis parvus OBBP]
ATCGCCGAATCTTTTGACTGCGGCGTCGCGGTATTGAACGACTGGCTACGCCGTCGCGCCATGCCGAACCAGCTCACCGG
CGCCTCGCGCACTTTCGTCATATCTCGCGACGAGCGCGTCGTCGGCTATTATGCGCTGGCTGCTGGCGCGATCGCCTCGC
ATGAGGCGCCCAGCCGCCTCAGTCGCAACATGCCCGATCCAATCCCGGTTTTCATACTTGGGCGCCTCGCCGTCGACCGA
TCGGAACAGGGTCGCATGCTCGGTTCGCTACTCCTGCGCGACGCGATCATCCGAACCCGCGCCGCCGCCGAATTCGGCGG
CATTGCCGGGCTGCTCGTCCATGCGCTCTCAGTCGAGGCCAAGCGCTTCTATATGCATCGCGGTTTCGTTGAAAGCCCTT
CAAGGCCCATGACTCTCCTCGCGCGGCTCAAGGACATTAAAGAATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT193420 WP_000806442.1 NZ_CP070162:976960-977301 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT193420 NZ_CP092968:3822074-3822367 [Methylocystis parvus OBBP]
ATGGCTCAGGCGACAAAACGAAACGAACTCTCCGGTCGTGGCCTCGTCAACGTCAGAATGGCTGCGGAGGACAGGAACAT
CATCGATCAGGCCGCCAGGATCGCCGGCAAGTCCCGTACGGAATTCATGGTGGAAGCGGCGCGCCGCGCGGCGCAGGATA
CCGTGCTCGACGCCAATCTGATCGTTGTGGACGGCAAGACTTTCGAACAGTTTCGCGCAATATTCGATGCGCCGGCGAAA
CCCAACGCCCATATTCAGAAACTCATGAATCTGAAGGCCCCGTGGGAGAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References