Detailed information of TA system TA193418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 746958..747652
• Replicon: chromosome
• Accession: NZ_CP070162
• Organism: Escherichia coli strain FDAARGOS_1283

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K13_RS03495
• Protein ID: WP_001263500.1
• Coordinates: 747254..747652 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K13_RS03490
• Protein ID: WP_000554758.1
• Coordinates: 746958..747251 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K13_RS03465 (742402)	742402..742899	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
I6K13_RS03470 (742894)	742894..743238	-	345	WP_125093574.1	hypothetical protein	-
I6K13_RS03475 (743317)	743317..745023	-	1707	Protein_689	flagellar biosynthesis protein FlhA	-
I6K13_RS03480 (744995)	744995..745780	+	786	WP_061301231.1	putative lateral flagellar export/assembly protein LafU	-
I6K13_RS03485 (745851)	745851..746906	+	1056	WP_001226168.1	DNA polymerase IV	-
I6K13_RS03490 (746958)	746958..747251	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K13_RS03495 (747254)	747254..747652	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K13_RS03500 (747662)	747662..748114	+	453	WP_071778924.1	GNAT family N-acetyltransferase	-
I6K13_RS03505 (748304)	748304..749443	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
I6K13_RS03510 (749440)	749440..750054	+	615	WP_000602123.1	peptide chain release factor H	-
I6K13_RS03515 (750111)	750111..751568	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K13_RS03520 (751829)	751829..752287	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	727340..748114	20774

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T193418 WP_001263500.1 NZ_CP070162:747254-747652 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193418 NZ_CP092968:2721861-2722361 [Methylocystis parvus OBBP]
GTGACGCTCGCGCCGCCGCGCCCGATATTGGAGACCGACGATCTGTCCGTTTTCGACTGCGGCTCCGTTGCGATGGACGA
ATGGCTGAAGAAACGCGCACGTCCAAATCAGGCGAGCGGGGCTTCTCGCACATATGTCGTCTGCAACGATGAGACGATCG
TCGCTTATTTCGCACTCGCTTCCGGCGCCGTCGATTTGGCGGAGGCGACCAACCGGCTGAAGCGCAACATGCCCGACCCC
GTGCCCGTGCCGGTCGCCGTCCTCGGGCGGCTCGCAATCGACCGAAGCTGGCAGAAGCAGGGTCTCGGGCGAGCGCTCGC
GAAGGACGCTTTCCAGCGCGTGCTCGCAGCGTCCAACGCGATCGGCATCCGGGGGATGCTCGTCCACGCAATATCCGCCG
AGGCCGCGGCTTTTTATCAAGCAACGGGATTTACGCCGTCGCCACTAGATCCCATGACGCTCATGGCGACGCTTATCGAT
CTACGCGCGGCCCTGGACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT193418 WP_000554758.1 NZ_CP070162:746958-747251 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193418 NZ_CP092968:2721583-2721864 [Methylocystis parvus OBBP]
ATGCAATCAAGATCCAACACAGCCAAGCGCGATACGCTCAATCTGCGGGTTAAGCCGGGCGATCGCGAACTGATCGATCG
TGCGGCCACGCTTGTCGGGAAGAGCCGGACGGATTTCCTGCTGGACGCCGGCCGGCGCGCGGCGCAGGACGCCTTGCTGG
ATCGCAGGTTGTGGCGCGTCGATTCGGCGGCTTTTGACGCTTTCGTCGCGCTGCTGGACGCCCCGCCGCAACCCAATGAG
CGGCTTCGCCGCACCATGAAGACGCGGGCTCCCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3