Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 98403..99130 Replicon plasmid unnamed1
Accession NZ_CP070159
Organism Klebsiella pneumoniae strain FDAARGOS_1310

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag I6K40_RS26215 Protein ID WP_004118600.1
Coordinates 98819..99130 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag I6K40_RS26210 Protein ID WP_000990392.1
Coordinates 98403..98822 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K40_RS26190 93885..95360 - 1476 WP_020324153.1 SEC-C domain-containing protein -
I6K40_RS26195 97062..97292 - 231 Protein_109 replication initiation protein -
I6K40_RS26200 97345..98049 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
I6K40_RS26205 98157..98366 + 210 WP_020806042.1 hypothetical protein -
I6K40_RS26210 98403..98822 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
I6K40_RS26215 98819..99130 - 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
I6K40_RS26220 99341..99721 + 381 Protein_114 gluconate permease -
I6K40_RS26225 99771..100466 - 696 WP_004118595.1 lactate utilization protein C -
I6K40_RS26230 100459..101886 - 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
I6K40_RS26235 101897..102616 - 720 WP_001102107.1 (Fe-S)-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / qnrB1 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa - 1..248395 248395
- flank IS/Tn - - 97345..98049 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T193413 WP_004118600.1 NZ_CP070159:c99130-98819 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T193413 NZ_CP092923:780985-781284 [Pseudomonas syringae pv. tagetis]
ATGACCACGATAAAACAGACCTCAACCTATATGGCTTGGGAACGCAGGCTCAAAGACCAAAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCGCCAGTAGGGCAGGGCGTGAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAACTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCCAAGAAACTGGCTGATCAATGGAGGCAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT193413 WP_000990392.1 NZ_CP070159:c98822-98403 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT193413 NZ_CP092923:781281-781565 [Pseudomonas syringae pv. tagetis]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGGCCTGAGGCAATTGCGATTTTTATCTCTGACGC
ATTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGACCGAGCTTGCGC
GTGAGACAGGTCTTTCTCGAGAGCAGCTTTACAGATCGTTCAGTAGCAAGGGCAATCCAACCTTAAAAACCATGTTGACG
GTAATGAGAGCCCTGGGCGTGGACATGACAGCTCGAATGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References