Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2135126..2135723 Replicon chromosome
Accession NZ_CP070158
Organism Klebsiella pneumoniae strain FDAARGOS_1310

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag I6K40_RS10140 Protein ID WP_004142563.1
Coordinates 2135126..2135443 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag I6K40_RS10145 Protein ID WP_004142561.1
Coordinates 2135436..2135723 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K40_RS10125 2130826..2132253 - 1428 WP_009308097.1 MFS transporter -
I6K40_RS10130 2132362..2133228 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
I6K40_RS10135 2133898..2134941 + 1044 WP_020325241.1 DUF2157 domain-containing protein -
I6K40_RS10140 2135126..2135443 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K40_RS10145 2135436..2135723 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
I6K40_RS10150 2135988..2136641 - 654 Protein_1966 oxygen-insensitive NAD(P)H nitroreductase -
I6K40_RS10155 2136761..2137129 - 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
I6K40_RS10160 2137129..2137710 - 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
I6K40_RS10165 2137890..2139005 + 1116 WP_020325248.1 MBL fold metallo-hydrolase -
I6K40_RS10170 2139036..2139377 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
I6K40_RS10175 2139395..2139643 - 249 WP_020325243.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T193402 WP_004142563.1 NZ_CP070158:2135126-2135443 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T193402 NZ_CP092911:c4254689-4254321 [Salmonella enterica subsp. enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGTCTGATCCTGGGCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATAGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT193402 WP_004142561.1 NZ_CP070158:2135436-2135723 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT193402 NZ_CP092911:c4254907-4254686 [Salmonella enterica subsp. enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References