Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4284117..4284811 Replicon chromosome
Accession NZ_CP070157
Organism Escherichia coli strain FDAARGOS_1276

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag I6K06_RS20380 Protein ID WP_001263491.1
Coordinates 4284413..4284811 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag I6K06_RS20375 Protein ID WP_000554755.1
Coordinates 4284117..4284410 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K06_RS20350 (4279738) 4279738..4280094 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
I6K06_RS20355 (4280087) 4280087..4280365 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
I6K06_RS20360 (4280470) 4280470..4282182 - 1713 Protein_4015 flagellar biosynthesis protein FlhA -
I6K06_RS20365 (4282154) 4282154..4282939 + 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
I6K06_RS20370 (4283010) 4283010..4284065 + 1056 WP_001550655.1 DNA polymerase IV -
I6K06_RS20375 (4284117) 4284117..4284410 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
I6K06_RS20380 (4284413) 4284413..4284811 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
I6K06_RS20385 (4284821) 4284821..4285273 + 453 WP_019842500.1 GNAT family N-acetyltransferase -
I6K06_RS20390 (4285519) 4285519..4285725 + 207 Protein_4021 RtcB family protein -
I6K06_RS20395 (4285721) 4285721..4286073 + 353 Protein_4022 peptide chain release factor H -
I6K06_RS20400 (4286130) 4286130..4287587 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
I6K06_RS20405 (4287848) 4287848..4288306 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4288902) 4288902..4288982 + 81 NuclAT_8 - -
- (4288902) 4288902..4288982 + 81 NuclAT_8 - -
- (4288902) 4288902..4288982 + 81 NuclAT_8 - -
- (4288902) 4288902..4288982 + 81 NuclAT_8 - -
I6K06_RS20410 (4288398) 4288398..4289642 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T193389 WP_001263491.1 NZ_CP070157:4284413-4284811 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T193389 NZ_CP092907:2717788-2717931 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACAATCCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT193389 WP_000554755.1 NZ_CP070157:4284117-4284410 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT193389 NZ_CP092907:c2717676-2717530 [Enterococcus faecalis]
TTGTGCTATAATGATAATGAAAAGAGAGCTATGCGCAAACATACCTCTCTGGTGTAGAGCCGTTTAAGACGGTGACCATG
TTAATTATTTAAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCTTCTTTAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References