Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3897369..3898168 Replicon chromosome
Accession NZ_CP070157
Organism Escherichia coli strain FDAARGOS_1276

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag I6K06_RS18570 Protein ID WP_000347270.1
Coordinates 3897369..3897833 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag I6K06_RS18575 Protein ID WP_001551693.1
Coordinates 3897833..3898168 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K06_RS18540 (3892370) 3892370..3892804 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
I6K06_RS18545 (3892822) 3892822..3893700 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K06_RS18550 (3893690) 3893690..3894469 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K06_RS18555 (3894480) 3894480..3894953 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K06_RS18560 (3894976) 3894976..3896256 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K06_RS18565 (3896505) 3896505..3897314 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
I6K06_RS18570 (3897369) 3897369..3897833 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K06_RS18575 (3897833) 3897833..3898168 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K06_RS18580 (3898317) 3898317..3899888 - 1572 WP_001273738.1 galactarate dehydratase -
I6K06_RS18585 (3900263) 3900263..3901597 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6K06_RS18590 (3901613) 3901613..3902383 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T193388 WP_000347270.1 NZ_CP070157:c3897833-3897369 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T193388 NZ_CP092907:2717161-2717358 [Enterococcus faecalis]
ATGTGTAGAGAAGTTATGGCGGTGGCTATCCAATCTGAAAGAGAGGTGATGCGTATGCACGTATTTCCGAAATTTAGAGA
AAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGATTGCGC
TGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT193388 WP_001551693.1 NZ_CP070157:c3898168-3897833 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT193388 NZ_CP092907:c2717477-2717291 [Enterococcus faecalis]
TGTGCTATAATGAAAAGGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTTT
TAGTTACAAAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References