Detailed information of TA system TA193386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3040453..3041055
• Replicon: chromosome
• Accession: NZ_CP070157
• Organism: Escherichia coli strain FDAARGOS_1276

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6K06_RS14520
• Protein ID: WP_000897305.1
• Coordinates: 3040744..3041055 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K06_RS14515
• Protein ID: WP_000356395.1
• Coordinates: 3040453..3040743 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K06_RS14480 (3036077)	3036077..3036979	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K06_RS14485 (3036976)	3036976..3037611	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K06_RS14490 (3037608)	3037608..3038537	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K06_RS14495 (3038719)	3038719..3038961	-	243	WP_001309881.1	CopG family transcriptional regulator	-
I6K06_RS14500 (3039180)	3039180..3039398	-	219	WP_001295676.1	CopG family transcriptional regulator	-
I6K06_RS14505 (3039817)	3039817..3040095	-	279	WP_001315112.1	hypothetical protein	-
I6K06_RS14510 (3040147)	3040147..3040368	-	222	WP_001550354.1	hypothetical protein	-
I6K06_RS14515 (3040453)	3040453..3040743	-	291	WP_000356395.1	NadS family protein	Antitoxin
I6K06_RS14520 (3040744)	3040744..3041055	-	312	WP_000897305.1	hypothetical protein	Toxin
I6K06_RS14525 (3041284)	3041284..3042192	+	909	WP_001550353.1	alpha/beta hydrolase	-
I6K06_RS24225 (3042360)	3042360..3043274	-	915	WP_109553727.1	transposase	-
I6K06_RS24230 (3043287)	3043287..3044174	-	888	Protein_2874	hypothetical protein	-
I6K06_RS14535 (3044590)	3044590..3045531	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K06_RS14540 (3045576)	3045576..3046013	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T193386 WP_000897305.1 NZ_CP070157:c3041055-3040744 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193386 NZ_CP092907:c2634845-2634702 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT193386 WP_000356395.1 NZ_CP070157:c3040743-3040453 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193386 NZ_CP092907:2634584-2634769 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TAGTTATAAAAAATAACCGTACCAGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCGC
AATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7