Detailed information of TA system TA193379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1929665..1930392
• Replicon: chromosome
• Accession: NZ_CP070157
• Organism: Escherichia coli strain FDAARGOS_1276

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: I6K06_RS09225
• Protein ID: WP_000547555.1
• Coordinates: 1930081..1930392 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K06_RS09220
• Protein ID: WP_000126294.1
• Coordinates: 1929665..1930084 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K06_RS09200 (1924750)	1924750..1925277	-	528	WP_001078777.1	electron transport protein HydN	-
I6K06_RS09205 (1925426)	1925426..1926436	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
I6K06_RS09210 (1926696)	1926696..1928153	+	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I6K06_RS09215 (1928162)	1928162..1929586	+	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
I6K06_RS09220 (1929665)	1929665..1930084	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I6K06_RS09225 (1930081)	1930081..1930392	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6K06_RS09230 (1930559)	1930559..1931029	-	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
I6K06_RS09235 (1931022)	1931022..1931432	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6K06_RS09240 (1931429)	1931429..1932196	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6K06_RS09245 (1932196)	1932196..1932738	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I6K06_RS09250 (1932748)	1932748..1934457	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T193379 WP_000547555.1 NZ_CP070157:c1930392-1930081 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T193379 NZ_CP092902:c90861-90379 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT193379 WP_000126294.1 NZ_CP070157:c1930084-1929665 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT193379 NZ_CP092902:c91130-90852 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: V0YLE2