Detailed information of TA system TA193363

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4928512..4928883
• Replicon: chromosome
• Accession: NZ_CP070152
• Organism: Escherichia coli strain FDAARGOS_1285

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: I6K15_RS23945
• Protein ID: WP_001317028.1
• Coordinates: 4928689..4928883 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4928512..4928690 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K15_RS25075 (4924264)	4924264..4924437	+	174	WP_001296046.1	protein YnaL	-
I6K15_RS23920 (4924467)	4924467..4925840	+	1374	WP_022645642.1	ATP-dependent RNA helicase DbpA	-
I6K15_RS23925 (4925969)	4925969..4926904	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I6K15_RS23930 (4926956)	4926956..4928191	-	1236	WP_000040858.1	site-specific integrase	-
I6K15_RS23935 (4928193)	4928193..4928408	-	216	WP_000079604.1	excisionase XisR	-
- (4928512)	4928512..4928690	+	179	NuclAT_0	-	Antitoxin
- (4928512)	4928512..4928690	+	179	NuclAT_0	-	Antitoxin
- (4928512)	4928512..4928690	+	179	NuclAT_0	-	Antitoxin
- (4928512)	4928512..4928690	+	179	NuclAT_0	-	Antitoxin
I6K15_RS23940 (4928487)	4928487..4928696	-	210	WP_063814942.1	double-strand break reduction protein RcbA	-
I6K15_RS23945 (4928689)	4928689..4928883	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I6K15_RS23950 (4928940)	4928940..4929749	-	810	WP_000166319.1	recombination protein RecT	-
I6K15_RS23955 (4929742)	4929742..4932342	-	2601	WP_000105124.1	exodeoxyribonuclease VIII	-
I6K15_RS23960 (4932444)	4932444..4932719	-	276	WP_000632297.1	protein RacC	-
I6K15_RS23965 (4932794)	4932794..4932964	-	171	WP_001352098.1	YdaE family protein	-
I6K15_RS23970 (4932964)	4932964..4933185	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4926956..4977629	50673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T193363 WP_001317028.1 NZ_CP070152:c4928883-4928689 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T193363 NZ_CP092892:c582378-582091 [Agrobacterium tumefaciens]
GTGCTGCCGCGCAGTTCGGATTTCACCAGGCAATTTATCAAGGACTGGCAACGGCTGAACAATTCCGGGCGTTATGACAT
GGTGAAACTGAAGGAGATCATGCTCCTTCTCATTGCCAACGAAGCGCCATTACCGGCGCAGTTTCGTGACCATGAATTGA
CCGGAGAATGGCACGATCATCGCGAATGCCATGTCGGCGGGGACTTTCTGTTGATCTACAAACTTGATGAGAAGCAGAAC
CTTCTCATCTTCACCCGCGCAGGAACGCACGCCGAGCTTTTCAGGTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT193363 NZ_CP070152:4928512-4928690 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTCAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin