Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3981511..3982190 Replicon chromosome
Accession NZ_CP070152
Organism Escherichia coli strain FDAARGOS_1285

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag I6K15_RS19535 Protein ID WP_000057541.1
Coordinates 3981511..3981813 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K15_RS19540 Protein ID WP_000806442.1
Coordinates 3981849..3982190 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K15_RS19515 (3976774) 3976774..3977994 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
I6K15_RS19520 (3978212) 3978212..3979864 + 1653 Protein_3714 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
I6K15_RS19525 (3979901) 3979901..3980380 - 480 WP_000186627.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K15_RS19530 (3980584) 3980584..3981378 - 795 WP_000365164.1 TraB/GumN family protein -
I6K15_RS19535 (3981511) 3981511..3981813 + 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K15_RS19540 (3981849) 3981849..3982190 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K15_RS19545 (3982248) 3982248..3984752 - 2505 WP_077737757.1 copper-exporting P-type ATPase CopA -
I6K15_RS19550 (3985015) 3985015..3985947 + 933 WP_000883035.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T193356 WP_000057541.1 NZ_CP070152:3981511-3981813 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T193356 NZ_CP092888:1592838-1593161 [Bifidobacterium sp. FKU]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGCCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT193356 WP_000806442.1 NZ_CP070152:3981849-3982190 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT193356 NZ_CP092888:1592612-1592851 [Bifidobacterium sp. FKU]
ATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCACCCCGGC
CGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAAATGACG
TGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References