Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3757570..3758264 Replicon chromosome
Accession NZ_CP070152
Organism Escherichia coli strain FDAARGOS_1285

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag I6K15_RS18490 Protein ID WP_001263491.1
Coordinates 3757866..3758264 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag I6K15_RS18485 Protein ID WP_000554755.1
Coordinates 3757570..3757863 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K15_RS18460 (3752606) 3752606..3752866 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
I6K15_RS18465 (3753052) 3753052..3753825 + 774 WP_022645219.1 C40 family peptidase -
I6K15_RS18470 (3753923) 3753923..3755635 - 1713 Protein_3506 flagellar biosynthesis protein FlhA -
I6K15_RS18475 (3755607) 3755607..3756392 + 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
I6K15_RS18480 (3756463) 3756463..3757518 + 1056 WP_022645222.1 DNA polymerase IV -
I6K15_RS18485 (3757570) 3757570..3757863 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
I6K15_RS18490 (3757866) 3757866..3758264 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
I6K15_RS18495 (3758274) 3758274..3758726 + 453 WP_023909048.1 GNAT family N-acetyltransferase -
I6K15_RS18500 (3758917) 3758917..3760056 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
I6K15_RS18505 (3760053) 3760053..3760667 + 615 WP_022645225.1 peptide chain release factor H -
I6K15_RS18510 (3760729) 3760729..3761238 + 510 WP_001361775.1 metal-dependent hydrolase -
I6K15_RS18515 (3761255) 3761255..3761536 - 282 WP_022645226.1 hypothetical protein -
I6K15_RS18520 (3761545) 3761545..3763002 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T193354 WP_001263491.1 NZ_CP070152:3757866-3758264 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T193354 NZ_CP092888:1134050-1134541 [Bifidobacterium sp. FKU]
ATGACCGACGTTTCCATGCAGCGTATTTTGCTCGATACCAATGTACTGTTGGACTATTTGCTCCATCGCGATGATCACGC
CAAGATGGCGGAAGCGGTTATGGAACTTGGCGCGAAGAACAACGTTACGTTGTTGTGCGCGTCATTGTCGCTGAAGGATA
TCGCATACCTATCATCATCGGCGATTCGTAGGGAATGCAAACCGAATGAATCGGAAGTGGAGAATTTCACTCGCGGCTTT
CTGTCGTCACGGGTGCCATGGCGTTGTATCGAGCAGGTGAAGGAGATGTGTGACATCGTCGCTGTCGATGAGTCGACATG
TGATAAGGCTTTTTCCCTGCAAAAACGGCATCGGGATTTTGAAGATGATCTGATCATCGTTGCCGCGCAACAATCCGGTG
CCAACTGTGTGGTCACATCGGATGCCGAATTAATCAGTCATTTTCCCGAATATTGCAGAACACCGGCTGAAATCGTGGCG
GCACTGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT193354 WP_000554755.1 NZ_CP070152:3757570-3757863 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT193354 NZ_CP092888:1133688-1134050 [Bifidobacterium sp. FKU]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTATACATCGCCGACCGCAAGGAAGTGCCGGCATTGGAAACGACCGCCG
AAGAGCAAGCCCGCGAGGAGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGTGCCGGATACGTGCACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGACAAAATGTACGA
CGAGCAGCTTGATACGTATTACAACATGAAGAGGGCCGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References