Detailed information of TA system TA193348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1959709..1960508
• Replicon: chromosome
• Accession: NZ_CP070152
• Organism: Escherichia coli strain FDAARGOS_1285

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: I6K15_RS10125
• Protein ID: WP_000347273.1
• Coordinates: 1960044..1960508 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: I6K15_RS10120
• Protein ID: WP_001308975.1
• Coordinates: 1959709..1960044 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K15_RS10105 (1955492)	1955492..1956264	-	773	Protein_1871	2-dehydro-3-deoxyglucarate aldolase	-
I6K15_RS10110 (1956280)	1956280..1957614	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
I6K15_RS10115 (1957989)	1957989..1959560	+	1572	WP_023908830.1	galactarate dehydratase	-
I6K15_RS10120 (1959709)	1959709..1960044	+	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6K15_RS10125 (1960044)	1960044..1960508	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6K15_RS10130 (1960563)	1960563..1961372	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6K15_RS10135 (1961621)	1961621..1962901	+	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6K15_RS10140 (1962924)	1962924..1963397	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6K15_RS10145 (1963408)	1963408..1964187	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
I6K15_RS10150 (1964177)	1964177..1965055	+	879	WP_204628439.1	PTS N-acetylgalactosamine transporter subunit IID	-
I6K15_RS10155 (1965073)	1965073..1965507	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T193348 WP_000347273.1 NZ_CP070152:1960044-1960508 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T193348 NZ_CP092862:147721-148119 [Salmonella enterica]
ATGCTGAAGTTTATGCTGGATACCAATATCTGTATCTTTACGATAAAAAACAAACCTGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGAAGAATGTGTATCAGTTCGGTTACCCTGATGGAGCTGATTTACGGTGCCGAAAAAAGCCAGATGCCGG
AACGAAATCTTGCTGTGATCGAGGGTTTTGTCTCCCGTCTTGATGTTCTGGATTATGACCCTGCGGCAGCCACTCATACC
GGGCAGATAAGAGCAGATCTTGCGCGTCAGGGACGTCCCGTCGGGCCATTCGATCAGATGATTGCCGGACACGCACGCAG
CCGGGGCCTGATTGTTGTCACGAACAACACCCGGGAGTTTGAACGTGTGGACGGACTGAGAACAGAGGACTGGAGCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT193348 WP_001308975.1 NZ_CP070152:1959709-1960044 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT193348 NZ_CP092862:147494-147721 [Salmonella enterica]
ATGGAAACCAGCGTATTTCTCAGTAACCGCAGCCAGGCTGTCAGACTGCCCAAAGCCGTTGCATTGCCGGAAAATGTAAA
ACGGGTTGAGATTATTGCCGTCGGGCGAACCCGTATCATTACGCCAGCCGGAGAATCGTGGGATGAATGGTTTGACGGGA
ACAACGTCAGCGCCGATTTTATGGATAACCGGGAACAACCCTCTGTGCAGGAACGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08