Detailed information of TA system TA193333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4878065..4878667
• Replicon: chromosome
• Accession: NZ_CP070148
• Organism: Escherichia coli strain FDAARGOS_1297

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K27_RS23435
• Protein ID: WP_000897302.1
• Coordinates: 4878065..4878376 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K27_RS23440
• Protein ID: WP_000356397.1
• Coordinates: 4878377..4878667 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K27_RS23410 (4873979)	4873979..4874578	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6K27_RS23415 (4874572)	4874572..4875444	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K27_RS23420 (4875441)	4875441..4875878	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K27_RS23425 (4875923)	4875923..4876864	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K27_RS23430 (4876928)	4876928..4877836	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6K27_RS23435 (4878065)	4878065..4878376	+	312	WP_000897302.1	hypothetical protein	Toxin
I6K27_RS23440 (4878377)	4878377..4878667	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6K27_RS23445 (4879026)	4879026..4879304	+	279	WP_001296612.1	hypothetical protein	-
I6K27_RS23450 (4879700)	4879700..4879918	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K27_RS23455 (4880134)	4880134..4881063	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K27_RS23460 (4881060)	4881060..4881695	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K27_RS23465 (4881692)	4881692..4882594	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T193333 WP_000897302.1 NZ_CP070148:4878065-4878376 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193333 NZ_CP092860:c4904781-4904407 [Citrobacter braakii]
ATGGTCACAGGATCTGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGCGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATCGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT193333 WP_000356397.1 NZ_CP070148:4878377-4878667 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193333 NZ_CP092860:c4905023-4904781 [Citrobacter braakii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCCGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: S1P416