Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2783912..2784414 Replicon chromosome
Accession NZ_CP070148
Organism Escherichia coli strain FDAARGOS_1297

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6K27_RS13435 Protein ID WP_000767822.1
Coordinates 2783912..2784166 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K27_RS13440 Protein ID WP_001259255.1
Coordinates 2784163..2784414 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K27_RS13415 (2780405) 2780405..2781763 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
I6K27_RS13420 (2781753) 2781753..2781815 - 63 WP_010723108.1 membrane protein YoeI -
I6K27_RS13425 (2782030) 2782030..2782959 - 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
I6K27_RS13430 (2783005) 2783005..2783829 - 825 WP_000754733.1 SDR family oxidoreductase -
I6K27_RS13435 (2783912) 2783912..2784166 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K27_RS13440 (2784163) 2784163..2784414 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K27_RS13445 (2784697) 2784697..2784747 + 51 WP_001364200.1 his operon leader peptide -
I6K27_RS13450 (2784893) 2784893..2785792 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K27_RS13455 (2785798) 2785798..2787102 + 1305 WP_001297912.1 histidinol dehydrogenase -
I6K27_RS13460 (2787099) 2787099..2788169 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
I6K27_RS13465 (2788169) 2788169..2789236 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T193330 WP_000767822.1 NZ_CP070148:c2784166-2783912 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T193330 NZ_CP092860:2535909-2536097 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGCTTACAGTCATCATCAATATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT193330 WP_001259255.1 NZ_CP070148:c2784414-2784163 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT193330 NZ_CP092860:2536129-2536545 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCGCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
TCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCGCTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCAACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACAATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References