Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2165691..2166253 Replicon chromosome
Accession NZ_CP070148
Organism Escherichia coli strain FDAARGOS_1297

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K27_RS10480 Protein ID WP_000605675.1
Coordinates 2165975..2166253 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K27_RS10475 Protein ID WP_000781370.1
Coordinates 2165691..2165975 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K27_RS10460 (2160872) 2160872..2163919 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
I6K27_RS10465 (2163932) 2163932..2164816 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K27_RS10470 (2164809) 2164809..2165462 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K27_RS10475 (2165691) 2165691..2165975 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K27_RS10480 (2165975) 2165975..2166253 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K27_RS10485 (2166439) 2166439..2167449 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K27_RS10490 (2167583) 2167583..2169280 - 1698 WP_000433462.1 malate dehydrogenase -
I6K27_RS10495 (2169436) 2169436..2169573 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K27_RS10500 (2169675) 2169675..2169890 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K27_RS10505 (2170235) 2170235..2170666 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T193319 WP_000605675.1 NZ_CP070148:c2166253-2165975 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T193319 NZ_CP092854:85724-86155 [Burkholderia contaminans]
ATGGCCTACCTTCTCGACGTCAACGTACTCATTGCTTTGCTCGACCCGGCACATGTTCAACACGAGCCTGCGCATGCATG
GTTTGGCCGGGTAGGCCATTCCGCATGGGCCACGTGCCCGCTCACCGAGAACGGCGTGCTTCGCATTATCGGTAATCCGA
AATACCCGAATACGATGGGCTCTCCGGCATCCGTTGCGCCGCTTGTTGCACAACTGCGGTCCCATGCCGGGCACACGTTC
TGGTCGGACGATGTGAGCCTGCTCGACGAAAAGCACGTCGACGCTACGCGCTTGCTGTCGTCTGCTCAGCTGACCGACAC
TTATCTCCTCGCGCTCGCGCGCGCGCACAAAGGCAAGTTGGCGACCTTCGACAAGCGCCTGGTTGTCGACGCCGTGCCGA
ATGGCGCGAAGCATCTTGAACTGATTTCTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT193319 WP_000781370.1 NZ_CP070148:c2165975-2165691 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT193319 NZ_CP092854:85497-85724 [Burkholderia contaminans]
ATGCGTACCACTATCGATATCGACGACGACGTCCTTGCCTTCGCGCGGGAGCGTGCAAAGCCCGGCACGAGTATCGGCAA
GGTCATTTCCGATCTCGCCCGTGCGGCTCTGCAGCGCGGGACAACGAACCAGTCGAGCCGGAACGGCTTGCCGTTGATGC
CGGTTTCCGCGACCGCCCGTCCGGTCACCCTTGATACCGTTAATCAACTTCGCGACGACTCGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References