Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1104948..1105627 Replicon chromosome
Accession NZ_CP070148
Organism Escherichia coli strain FDAARGOS_1297

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag I6K27_RS05195 Protein ID WP_000057524.1
Coordinates 1104948..1105250 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K27_RS05200 Protein ID WP_000806442.1
Coordinates 1105286..1105627 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K27_RS05170 (1100324) 1100324..1101976 + 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
I6K27_RS05175 (1102014) 1102014..1102517 - 504 WP_000667000.1 hypothetical protein -
I6K27_RS05180 (1102514) 1102514..1103314 - 801 WP_000439796.1 hypothetical protein -
I6K27_RS05185 (1103338) 1103338..1103817 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K27_RS05190 (1104021) 1104021..1104815 - 795 WP_000365177.1 TraB/GumN family protein -
I6K27_RS05195 (1104948) 1104948..1105250 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K27_RS05200 (1105286) 1105286..1105627 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K27_RS05205 (1105685) 1105685..1108189 - 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
I6K27_RS05210 (1108451) 1108451..1109383 + 933 WP_000883052.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1102014..1114228 12214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T193313 WP_000057524.1 NZ_CP070148:1104948-1105250 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T193313 NZ_CP092852:470894-471310 [Burkholderia contaminans]
GTGTATTTGATTGACACGAATATCATCAGCGAAATCAGGAAGGGCAAGCGAACCAACCGTGGCGTGCGGGCGTTTTTCAG
GCAGGCCGCGGCGGACGCAAGCCCGCTTTATCTTTCCGTCGTAACGGTTGCTGAACTTCGCCGCGGCGTCGATCTGATCC
GTCATCGTGGCGATCATCCCCAGGCGTCGGCGCTCGAGGCCTGGATGGCGACGATCCTGTCCGGCTATGCGCCGAACATC
CTGCCGGTCGACATCGAGATCAGCCAGATGTGGGGGCATTTGCGCGTGCCCGATCCGACCCACGAACTCGACAAGCTGAT
CGCGGCCACCGCGCTGATCAACGATCTCACGGTGGTCACGCGCAACGTCGAGGATTTCGCCCGCACCGGCGTCAAGCTGC
TGAACCCGTTCGACTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT193313 WP_000806442.1 NZ_CP070148:1105286-1105627 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT193313 NZ_CP092852:470671-470907 [Burkholderia contaminans]
ATGGCAAATCTACTGGTGCGTAACGTGGATGACAGTATCGTTCAGAGCCTGCGCGAGCAGGCTGCGGCAAATGGCAGGAG
TGCCGAGGCCGAACATCGGGCCATCCTGGCCGATGCGCTCGGCCGGCCGAAGCGGAAGACATTTGCGCAAGTGCTGATGA
GCATGCCCGATGTTGGCGACGACGCGGATTTTCAACGTGTTCAGGATTCCGGCGAGGCCAGGCGTGTATTTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References