Detailed information of TA system TA193311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 876021..876715
• Replicon: chromosome
• Accession: NZ_CP070148
• Organism: Escherichia coli strain FDAARGOS_1297

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K27_RS04135
• Protein ID: WP_001263500.1
• Coordinates: 876317..876715 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K27_RS04130
• Protein ID: WP_000554758.1
• Coordinates: 876021..876314 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K27_RS04105 (871557)	871557..872054	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
I6K27_RS04110 (872137)	872137..872295	-	159	WP_014639450.1	hypothetical protein	-
I6K27_RS04115 (872374)	872374..874086	-	1713	Protein_813	flagellar biosynthesis protein FlhA	-
I6K27_RS04120 (874058)	874058..874843	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K27_RS04125 (874914)	874914..875969	+	1056	WP_001226168.1	DNA polymerase IV	-
I6K27_RS04130 (876021)	876021..876314	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K27_RS04135 (876317)	876317..876715	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K27_RS04140 (876725)	876725..877177	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K27_RS04145 (877367)	877367..878506	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K27_RS04150 (878503)	878503..879117	+	615	WP_000602123.1	peptide chain release factor H	-
I6K27_RS04155 (879174)	879174..880631	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K27_RS04160 (880892)	880892..881350	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	856496..877177	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T193311 WP_001263500.1 NZ_CP070148:876317-876715 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193311 NZ_CP092851:1854200-1854439 [Burkholderia contaminans]
ATTTGGCGCGATACCGTCGGGCGTGGGTCGCTCAAGCAAACCGACCTTTACCACCGCGATCTGATCGGAACCATGGAAGC
GCTGGCGCGCGGTGAAAAGGTCGGCCGGACTTGCATTGTTCGCGATGGATATTTCCGGTACGCCGTGGGCTCCCACGTCG
TGTTCTATCGTGAAACGGAATACACGATCGATTTGATTCGGGTCCTGCATCAACGCATGGACATCGAACAACATCTGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT193311 WP_000554758.1 NZ_CP070148:876021-876314 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193311 NZ_CP092851:1853909-1854154 [Burkholderia contaminans]
ATGTCGCAAAACGCATCCGTTTCCCGGCGCGACCACTTTGCCGAACTCGTCGATACCCAAGTGCAATCGGAACAATACGG
TTCCGCAAGCGACGTCGAACGAACCGGTTTGCGGCTTCTCGAATCACGCGACATGCAGGTGCGCGCGTTGCAGGAAGCGT
TGAAGGCCGGGGAGGTATCGGGAGCACTCAGGCCATTCGACCGCGAGGCCTTCCTGGCCCGCATGCGGGCCGCGCATGAC
GGCTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3