Detailed information of TA system TA193297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2786535..2787185
• Replicon: chromosome
• Accession: NZ_CP070144
• Organism: Klebsiella oxytoca strain FDAARGOS_1331

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6K61_RS12930
• Protein ID: WP_049128706.1
• Coordinates: 2786844..2787185 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K61_RS12925
• Protein ID: WP_017145203.1
• Coordinates: 2786535..2786834 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K61_RS12905	2782421..2783140	-	720	WP_004107085.1	phosphonate utilization transcriptional regulator PhnR	-
I6K61_RS12910	2783281..2784384	+	1104	WP_049593719.1	2-aminoethylphosphonate--pyruvate transaminase	-
I6K61_RS12915	2784394..2785203	+	810	WP_032729158.1	phosphonoacetaldehyde hydrolase	-
I6K61_RS12920	2785235..2786416	-	1182	WP_017145204.1	purine ribonucleoside efflux pump NepI	-
I6K61_RS12925	2786535..2786834	-	300	WP_017145203.1	helix-turn-helix transcriptional regulator	Antitoxin
I6K61_RS12930	2786844..2787185	-	342	WP_049128706.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K61_RS12935	2787437..2787889	-	453	WP_004116616.1	DUF1198 domain-containing protein	-
I6K61_RS12940	2788067..2789458	-	1392	WP_004107098.1	hexose-6-phosphate:phosphate antiporter	-
I6K61_RS12945	2789604..2790923	-	1320	WP_004107101.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13064.90 Da
• Isoelectric Point: 5.7456

>T193297 WP_049128706.1 NZ_CP070144:c2787185-2786844 [Klebsiella oxytoca]
MWNVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPQLGRPFADTVNDSAFSNMKELRVQHQGSPIRAFFVFDPSRRGI
LLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Nucleotide

• Length: 342 bp

>T193297 NZ_CP092844:c1323686-1323390 [Burkholderia ambifaria]
ATGGAAAAGAGAACGCCTCACTGCAAGCTGTCCCGGGTCAAGGCACTCGTCGAGGCCGGCAACGTTCGGATGACGACGAG
TGCGGTATTGGGCGCCCGGCAACTGGGTTTTACGCCAAACGAGGCAATCGGTATCGTAATGTCGCTGGACACCGCGGATT
TCTACAAGAGCATGACGACCTACGCCGACCACACGATCTGGCAGGACGTCTATTGGCGCCGCACGGTGAACGGCGACGTG
TATCTGAAACTGACCGTGATCGACGATGTGCTGATCGTGTCTTTCAAGGAGCGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10874.80 Da
• Isoelectric Point: 10.0948

>AT193297 WP_017145203.1 NZ_CP070144:c2786834-2786535 [Klebsiella oxytoca]
MATLKELMAKQSPESQARIAEKVEAMRQVIALSMLREELNLSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPGGKRIAFHL

Nucleotide

• Length: 300 bp

>AT193297 NZ_CP092844:c1323387-1322986 [Burkholderia ambifaria]
ATGAAGTGTCCTGCCTGCGGCGCCGCGAAGCTGATTCGGGATACACGAGACATTCCGTACACGTACAAGGGTCGCTCGAC
CGTCGTTCGCGATATAACCGGCGACTTCTGTCCGGCCTGCGGCGAGTCGGTGCTCGATATCGACGAGGCAACCCGGCTCG
GTGAAGCGATCGGCGAGTTCAACAAACAAGTGAATTCGACCATCGTCGACCCGAGGTTCATCGCGAAGGTGCGCAAGAAG
CTGATGCTGGATCAACGCGAGGCGGCCGAGATCTTCGGTGGCGGCGTCAATGCCTTCTCTCGCTACGAAACCGGCAAGAC
GAACCCCCCGCTTGCCCTGGTGAAGCTGCTCAAGATTCTGGATCGTCATCCGGATCTGCTCGCGGAAGTGCGCGCAGCGT
AG