Detailed information of TA system TA193286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3935363..3936057
• Replicon: chromosome
• Accession: NZ_CP070134
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: I6K11_RS19805
• Protein ID: WP_001521903.1
• Coordinates: 3935659..3936057 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K11_RS19800
• Protein ID: WP_000554758.1
• Coordinates: 3935363..3935656 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS19775 (3930478)	3930478..3931227	+	750	WP_016233951.1	C40 family peptidase	-
I6K11_RS19780 (3931284)	3931284..3931637	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
I6K11_RS19785 (3931716)	3931716..3933428	-	1713	Protein_3703	flagellar biosynthesis protein FlhA	-
I6K11_RS19790 (3933400)	3933400..3934185	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
I6K11_RS19795 (3934256)	3934256..3935311	+	1056	WP_001226177.1	DNA polymerase IV	-
I6K11_RS19800 (3935363)	3935363..3935656	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K11_RS19805 (3935659)	3935659..3936057	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K11_RS19810 (3936067)	3936067..3936519	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
I6K11_RS19815 (3936764)	3936764..3936970	+	207	Protein_3709	RtcB family protein	-
I6K11_RS19820 (3936966)	3936966..3937318	+	353	Protein_3710	peptide chain release factor H	-
I6K11_RS19825 (3937375)	3937375..3938832	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
I6K11_RS19830 (3939093)	3939093..3939551	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (3940147)	3940147..3940227	+	81	NuclAT_11	-	-
- (3940147)	3940147..3940227	+	81	NuclAT_11	-	-
- (3940147)	3940147..3940227	+	81	NuclAT_11	-	-
- (3940147)	3940147..3940227	+	81	NuclAT_11	-	-
I6K11_RS19835 (3939643)	3939643..3940887	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T193286 WP_001521903.1 NZ_CP070134:3935659-3936057 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193286 NZ_CP092832:1953881-1954138 [Flavobacterium sp. HJ-32-4]
ATGCGGTACGTTTTTGTAGACGAATCTTGGGAAGACTACCTTTATTGGCAAAAAATCGATAAAAAGAAGGTAAAGCGAAT
CAACGACCTACTGAAGGATATTGCGCGGTCGCCTTTCGAAGGAATCGGAAAACCGGAACCCCTAAAACACAAATACTCCG
GATTCTGGTCGAGGCGGATCGATGAAGAGCACCGATTGATCTATCGCTACCAGGACGATGAAATCCAAATCGTCAAATGC
AGGCATCATTACGATTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT193286 WP_000554758.1 NZ_CP070134:3935363-3935656 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193286 NZ_CP092832:1953627-1953881 [Flavobacterium sp. HJ-32-4]
ATGTTCGTCGCCAATATCTCCGAATTCAGAAAAGACATCAAATCCTACCTTGATCGTGTTGCAAAGAATTTTGAAACCCT
GATCATCAACAGGGGTAAGGATTCCGGTATCGTTGTCATGTCGCTTGAAGAGTATAATTCCCTCATGGCCACCAGTTATG
AGTTGTCTTCCCGTGCGAACGAAAAAAGACTGGATTCGGCAATCGAGAAGCTGCGCAACGGGAAGGCATTCGATAAAGAC
CTGATTGAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3