Detailed information of TA system TA193280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2972894..2973496
• Replicon: chromosome
• Accession: NZ_CP070134
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6K11_RS15240
• Protein ID: WP_000897305.1
• Coordinates: 2972894..2973205 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K11_RS15245
• Protein ID: WP_000356395.1
• Coordinates: 2973206..2973496 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS15220 (2967935)	2967935..2968372	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K11_RS15225 (2968417)	2968417..2969358	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K11_RS27665 (2969774)	2969774..2970661	+	888	Protein_2806	hypothetical protein	-
I6K11_RS27670 (2970674)	2970674..2971588	+	915	WP_077634137.1	transposase	-
I6K11_RS15235 (2971757)	2971757..2972665	-	909	WP_001306651.1	alpha/beta hydrolase	-
I6K11_RS15240 (2972894)	2972894..2973205	+	312	WP_000897305.1	hypothetical protein	Toxin
I6K11_RS15245 (2973206)	2973206..2973496	+	291	WP_000356395.1	NadS family protein	Antitoxin
I6K11_RS15250 (2973581)	2973581..2973793	+	213	WP_000197769.1	hypothetical protein	-
I6K11_RS15255 (2973855)	2973855..2974133	+	279	WP_001306650.1	hypothetical protein	-
I6K11_RS15260 (2974552)	2974552..2974770	+	219	WP_001295676.1	CopG family transcriptional regulator	-
I6K11_RS15265 (2974989)	2974989..2975231	+	243	WP_021523315.1	hypothetical protein	-
I6K11_RS15270 (2975413)	2975413..2976342	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I6K11_RS15275 (2976339)	2976339..2976974	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K11_RS15280 (2976971)	2976971..2977873	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T193280 WP_000897305.1 NZ_CP070134:2972894-2973205 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193280 NZ_CP070134:2972894-2973205 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT193280 WP_000356395.1 NZ_CP070134:2973206-2973496 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193280 NZ_CP070134:2973206-2973496 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7