Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2139580..2140379 Replicon chromosome
Accession NZ_CP070134
Organism Escherichia coli strain FDAARGOS_1281

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag I6K11_RS11325 Protein ID WP_000347272.1
Coordinates 2139915..2140379 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag I6K11_RS11320 Protein ID WP_001307405.1
Coordinates 2139580..2139915 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K11_RS11305 (2135365) 2135365..2136135 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6K11_RS11310 (2136151) 2136151..2137485 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6K11_RS11315 (2137860) 2137860..2139431 + 1572 WP_001273738.1 galactarate dehydratase -
I6K11_RS11320 (2139580) 2139580..2139915 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K11_RS11325 (2139915) 2139915..2140379 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K11_RS11330 (2140434) 2140434..2141243 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I6K11_RS11335 (2141492) 2141492..2142772 + 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K11_RS11340 (2142795) 2142795..2143268 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K11_RS11345 (2143279) 2143279..2144058 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K11_RS11350 (2144048) 2144048..2144926 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K11_RS11355 (2144944) 2144944..2145378 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2130055..2140379 10324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T193279 WP_000347272.1 NZ_CP070134:2139915-2140379 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T193279 NZ_CP092828:3721172-3721459 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAATAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATAGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT193279 WP_001307405.1 NZ_CP070134:2139580-2139915 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT193279 NZ_CP092828:3721446-3721748 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGCCGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References