Detailed information of TA system TA193274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1597776..1598503
• Replicon: chromosome
• Accession: NZ_CP070134
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: I6K11_RS08785
• Protein ID: WP_001521139.1
• Coordinates: 1598192..1598503 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K11_RS08780
• Protein ID: WP_000126294.1
• Coordinates: 1597776..1598195 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS08760 (1592861)	1592861..1593388	-	528	WP_001078777.1	electron transport protein HydN	-
I6K11_RS08765 (1593537)	1593537..1594547	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
I6K11_RS08770 (1594807)	1594807..1596264	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I6K11_RS08775 (1596273)	1596273..1597697	+	1425	WP_204682314.1	6-phospho-beta-glucosidase	-
I6K11_RS08780 (1597776)	1597776..1598195	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I6K11_RS08785 (1598192)	1598192..1598503	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6K11_RS08790 (1598670)	1598670..1599140	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I6K11_RS08795 (1599133)	1599133..1599543	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6K11_RS08800 (1599540)	1599540..1600307	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6K11_RS08805 (1600307)	1600307..1600849	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I6K11_RS08810 (1600859)	1600859..1602568	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T193274 WP_001521139.1 NZ_CP070134:c1598503-1598192 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T193274 NZ_CP092828:c951543-951211 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT193274 WP_000126294.1 NZ_CP070134:c1598195-1597776 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT193274 NZ_CP092828:c951801-951544 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Structures

Toxin

• AlphaFold DB: A0A829L2T9