Detailed information of TA system TA193255

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91013..91267
• Replicon: plasmid unnamed2
• Accession: NZ_CP070133
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I6K11_RS00650
• Protein ID: WP_001312851.1
• Coordinates: 91118..91267 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	91013..91074 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS00615 (86665)	86665..86877	+	213	WP_005012601.1	hypothetical protein	-
I6K11_RS00620 (87178)	87178..87267	-	90	Protein_107	IS1 family transposase	-
I6K11_RS00625 (87322)	87322..87999	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
I6K11_RS00630 (87999)	87999..88346	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
I6K11_RS00635 (88366)	88366..89937	+	1572	WP_029392005.1	IS66-like element ISCro1 family transposase	-
I6K11_RS00640 (89975)	89975..90591	-	617	Protein_111	IS1-like element IS1A family transposase	-
I6K11_RS00645 (90692)	90692..90874	+	183	WP_000968309.1	hypothetical protein	-
- (91013)	91013..91074	-	62	NuclAT_1	-	Antitoxin
- (91013)	91013..91074	-	62	NuclAT_1	-	Antitoxin
- (91013)	91013..91074	-	62	NuclAT_1	-	Antitoxin
- (91013)	91013..91074	-	62	NuclAT_1	-	Antitoxin
I6K11_RS00650 (91118)	91118..91267	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
I6K11_RS00655 (91551)	91551..91808	+	258	WP_000083833.1	replication regulatory protein RepA	-
I6K11_RS00660 (92044)	92044..92118	+	75	WP_032336874.1	RepA leader peptide Tap	-
I6K11_RS00665 (92111)	92111..92557	+	447	Protein_116	plasmid replication initiator RepA	-
I6K11_RS00670 (92557)	92557..93171	-	615	Protein_117	VENN motif pre-toxin domain-containing protein	-
I6K11_RS00675 (93878)	93878..95098	+	1221	WP_000410951.1	arginine deiminase	-
I6K11_RS00680 (95109)	95109..96020	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / dfrA17 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / mph(A) / tet(B)	-	1..151845	151845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T193255 WP_001312851.1 NZ_CP070133:91118-91267 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T193255 NZ_CP092798:1962-2276 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT193255 NZ_CP070133:c91074-91013 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin