Detailed information of TA system TA193252

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50931..51200
• Replicon: plasmid unnamed2
• Accession: NZ_CP070133
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K11_RS00420
• Protein ID: WP_001372321.1
• Coordinates: 51075..51200 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	50931..50996 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS27520	46006..46254	+	249	WP_071606928.1	hypothetical protein	-
I6K11_RS00390	46724..47251	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
I6K11_RS00395	47307..47540	+	234	WP_000006018.1	DUF905 domain-containing protein	-
I6K11_RS00400	47599..49557	+	1959	WP_032152921.1	ParB/RepB/Spo0J family partition protein	-
I6K11_RS00405	49612..50046	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
I6K11_RS00410	50043..50805	+	763	Protein_62	plasmid SOS inhibition protein A	-
I6K11_RS00415	50774..50962	-	189	WP_001299721.1	hypothetical protein	-
-	50774..50998	+	225	NuclAT_0	-	-
-	50774..50998	+	225	NuclAT_0	-	-
-	50774..50998	+	225	NuclAT_0	-	-
-	50774..50998	+	225	NuclAT_0	-	-
-	50931..50996	-	66	-	-	Antitoxin
I6K11_RS27525	50984..51133	+	150	Protein_64	plasmid maintenance protein Mok	-
I6K11_RS00420	51075..51200	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K11_RS27530	51420..51650	+	231	WP_071587244.1	hypothetical protein	-
I6K11_RS27535	51648..51821	-	174	Protein_67	hypothetical protein	-
I6K11_RS00425	52119..52406	+	288	WP_000107537.1	hypothetical protein	-
I6K11_RS00430	52526..53347	+	822	WP_001234469.1	DUF932 domain-containing protein	-
I6K11_RS00435	53644..54246	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
I6K11_RS00440	54567..54950	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K11_RS00445	55137..55826	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / dfrA17 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / mph(A) / tet(B)	-	1..151845	151845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T193252 WP_001372321.1 NZ_CP070133:51075-51200 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T193252 NZ_CP092797:69-365 [Klebsiella pneumoniae]
ATGATTGAAATCAGGCAAACAGAAGAAGTTAAAAAATGGCTTAAAGGATTAAAAGATAAAATAGCCAAAGCAAAAATTTT
AACACGGATTGAGCGGATGAAAGAGGGGAATTATGGAGATGTTGAGCCGATAGGTGATGGATATTCAGAGTTAAGAATCC
ATCAGGGTAAGGGTTACAGAGTTTATTTTGCCAACCGAAACAATGAAATTATTTTATTGCTTTGTGGCGGTGATAAAACA
ACACAACAAGCAGACATAAAAAAAGCAAAACAGATAGCGAAAAAATGGGGGTTCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT193252 NZ_CP070133:c50996-50931 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin