Detailed information of TA system TA193247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4656703..4657397
• Replicon: chromosome
• Accession: NZ_CP070124
• Organism: Escherichia coli strain FDAARGOS_1292

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K22_RS22390
• Protein ID: WP_001263500.1
• Coordinates: 4656703..4657101 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K22_RS22395
• Protein ID: WP_000554758.1
• Coordinates: 4657104..4657397 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K22_RS22365 (4652068)	4652068..4652526	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
I6K22_RS22370 (4652787)	4652787..4654244	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K22_RS22375 (4654301)	4654301..4654915	-	615	WP_000602123.1	peptide chain release factor H	-
I6K22_RS22380 (4654912)	4654912..4656051	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K22_RS22385 (4656241)	4656241..4656693	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K22_RS22390 (4656703)	4656703..4657101	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K22_RS22395 (4657104)	4657104..4657397	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K22_RS22400 (4657449)	4657449..4658504	-	1056	WP_001226168.1	DNA polymerase IV	-
I6K22_RS22405 (4658575)	4658575..4659360	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K22_RS22410 (4659332)	4659332..4661044	+	1713	Protein_4422	flagellar biosynthesis protein FlhA	-
I6K22_RS22415 (4661123)	4661123..4661281	+	159	WP_014639450.1	hypothetical protein	-
I6K22_RS22420 (4661364)	4661364..4661861	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	4656703..4677057	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T193247 WP_001263500.1 NZ_CP070124:c4657101-4656703 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193247 NZ_CP092794:19053-19469 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT193247 WP_000554758.1 NZ_CP070124:c4657397-4657104 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193247 NZ_CP092794:18826-19056 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3