Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3430961..3431523 Replicon chromosome
Accession NZ_CP070124
Organism Escherichia coli strain FDAARGOS_1292

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K22_RS16350 Protein ID WP_000605675.1
Coordinates 3430961..3431239 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K22_RS16355 Protein ID WP_000781370.1
Coordinates 3431239..3431523 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K22_RS16325 (3426548) 3426548..3426979 - 432 WP_000152310.1 peroxiredoxin OsmC -
I6K22_RS16330 (3427324) 3427324..3427539 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K22_RS16335 (3427641) 3427641..3427778 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K22_RS16340 (3427934) 3427934..3429631 + 1698 WP_000433462.1 malate dehydrogenase -
I6K22_RS16345 (3429765) 3429765..3430775 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K22_RS16350 (3430961) 3430961..3431239 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K22_RS16355 (3431239) 3431239..3431523 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K22_RS16360 (3431752) 3431752..3432405 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K22_RS16365 (3432398) 3432398..3433282 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K22_RS16370 (3433295) 3433295..3436342 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T193244 WP_000605675.1 NZ_CP070124:3430961-3431239 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T193244 NZ_CP092793:c4909736-4909452 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT193244 WP_000781370.1 NZ_CP070124:3431239-3431523 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT193244 NZ_CP092793:c4909968-4909726 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References