Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2813424..2813926 Replicon chromosome
Accession NZ_CP070124
Organism Escherichia coli strain FDAARGOS_1292

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag I6K22_RS13405 Protein ID WP_000767826.1
Coordinates 2813672..2813926 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K22_RS13400 Protein ID WP_001259255.1
Coordinates 2813424..2813675 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K22_RS13375 (2808602) 2808602..2809669 - 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6K22_RS13380 (2809669) 2809669..2810739 - 1071 WP_000108997.1 histidinol-phosphate transaminase -
I6K22_RS13385 (2810736) 2810736..2812040 - 1305 WP_001350692.1 histidinol dehydrogenase -
I6K22_RS13390 (2812046) 2812046..2812945 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K22_RS13395 (2813091) 2813091..2813141 - 51 WP_001364200.1 his operon leader peptide -
I6K22_RS13400 (2813424) 2813424..2813675 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K22_RS13405 (2813672) 2813672..2813926 + 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K22_RS13410 (2814009) 2814009..2814833 + 825 WP_000754737.1 SDR family oxidoreductase -
I6K22_RS13415 (2814879) 2814879..2815808 + 930 WP_000803363.1 LysR substrate-binding domain-containing protein -
I6K22_RS13420 (2816023) 2816023..2816085 + 63 WP_010723108.1 membrane protein YoeI -
I6K22_RS13425 (2816075) 2816075..2817433 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T193233 WP_000767826.1 NZ_CP070124:2813672-2813926 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T193233 NZ_CP092790:27377-27673 [Klebsiella pneumoniae]
ATGATTGAAATCAGGCAAACAGAAGAAGTTAAAAAATGGCTTAAAGGATTAAAAGATAAAATAGCCAAAGCAAAAATTTT
AACACGGATTGAGCGGATGAAAGAGGGGAATTATGGAGATGTTGAGCCGATAGGTGATGGATATTCAGAGTTAAGAATCC
ATCAGGGTAAGGGTTACAGAGTTTATTTTGCCAACCGAAACAATGAAATTATTTTATTGCTTTGTGGCGGTGATAAAACA
ACACAACAAGCAGACATAAAAAAAGCAAAACAGATAGCGAAAAAATGGGGGTTCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT193233 WP_001259255.1 NZ_CP070124:2813424-2813675 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT193233 NZ_CP092790:27675-27959 [Klebsiella pneumoniae]
ATGAAAACAAAACCAGAATTATTTGATGTAGTCGATTACCTAGAGGATGAAGAAGATATTCAGGCATATCTCAATGCCGC
GCTGGATGAAGATGATGAAAGCTACTTTTTAGCAGCACTGGGAAATATTGCCAGAGCAAGAAATATCAGCCAGCTTTCAA
AAGAAACAGGAATGAGCCGTGAGGGAATTTATAAAGCTCTTTCCGGAACAGGAAACCCATCTTTTAAAACGATCTCAAAA
ATATCTAAAGCATTAGGTTTGAAAATACACTTCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References