Detailed information of TA system TA193229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 698054..698656
• Replicon: chromosome
• Accession: NZ_CP070124
• Organism: Escherichia coli strain FDAARGOS_1292

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K22_RS03390
• Protein ID: WP_000897302.1
• Coordinates: 698345..698656 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K22_RS03385
• Protein ID: WP_000356397.1
• Coordinates: 698054..698344 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K22_RS03360 (694127)	694127..695029	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K22_RS03365 (695026)	695026..695661	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K22_RS03370 (695658)	695658..696587	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K22_RS03375 (696803)	696803..697021	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K22_RS03380 (697417)	697417..697695	-	279	WP_001296612.1	hypothetical protein	-
I6K22_RS03385 (698054)	698054..698344	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K22_RS03390 (698345)	698345..698656	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K22_RS03395 (698885)	698885..699793	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6K22_RS03400 (699857)	699857..700798	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K22_RS03405 (700843)	700843..701280	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K22_RS03410 (701277)	701277..702149	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K22_RS03415 (702143)	702143..702742	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T193229 WP_000897302.1 NZ_CP070124:c698656-698345 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193229 NZ_CP092787:c84563-84093 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT193229 WP_000356397.1 NZ_CP070124:c698344-698054 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193229 NZ_CP092787:c85003-84560 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: S1P416