Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3101..3747 Replicon plasmid unnamed2
Accession NZ_CP070117
Organism Escherichia coli strain FDAARGOS_1298

Toxin (Protein)


Gene name higB Uniprot ID F4T8Q4
Locus tag I6K28_RS25525 Protein ID WP_000269926.1
Coordinates 3397..3747 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID F4T8Q5
Locus tag I6K28_RS25520 Protein ID WP_001259443.1
Coordinates 3101..3400 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K28_RS25490 (I6K28_25435) 323..595 + 273 WP_001272119.1 hypothetical protein -
I6K28_RS25495 (I6K28_25440) 669..968 + 300 WP_000113288.1 hypothetical protein -
I6K28_RS25500 (I6K28_25445) 1339..1872 + 534 WP_001025389.1 J domain-containing protein -
I6K28_RS25505 (I6K28_25450) 2000..2542 + 543 WP_000206647.1 hypothetical protein -
I6K28_RS25510 (I6K28_25455) 2578..2835 + 258 WP_000845367.1 hypothetical protein -
I6K28_RS25515 (I6K28_25460) 2825..3070 + 246 WP_001193619.1 hypothetical protein -
I6K28_RS25520 (I6K28_25465) 3101..3400 - 300 WP_001259443.1 XRE family transcriptional regulator Antitoxin
I6K28_RS25525 (I6K28_25470) 3397..3747 - 351 WP_000269926.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K28_RS25530 (I6K28_25475) 4113..4760 + 648 WP_000214917.1 ParA family protein -
I6K28_RS25535 (I6K28_25480) 4800..5033 + 234 WP_000024515.1 hypothetical protein -
I6K28_RS25540 (I6K28_25485) 5065..5328 - 264 WP_001312455.1 PilI type IV pilus biogenesis protein -
I6K28_RS26615 5507..5812 - 306 Protein_11 hypothetical protein -
I6K28_RS25550 (I6K28_25495) 5822..6286 - 465 WP_000697615.1 thermonuclease family protein -
I6K28_RS25555 (I6K28_25500) 6290..7024 - 735 WP_000905147.1 MobC family replication-relaxation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..24413 24413


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13742.77 Da        Isoelectric Point: 7.4217

>T193223 WP_000269926.1 NZ_CP070117:c3747-3397 [Escherichia coli]
MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHADTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAI
ILCAGDKSNDKKFYDRMIRIADEEFSTHLAELEGKK

Download         Length: 351 bp

>T193223 NZ_CP092786:c4807597-4807064 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10917.70 Da        Isoelectric Point: 4.6283

>AT193223 WP_001259443.1 NZ_CP070117:c3400-3101 [Escherichia coli]
MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFNI

Download         Length: 300 bp

>AT193223 NZ_CP092786:c4807874-4807608 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A743U745

Antitoxin

Source ID Structure
AlphaFold DB A0A743U677

References