Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3642533..3643035 Replicon chromosome
Accession NZ_CP070115
Organism Escherichia coli strain FDAARGOS_1298

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6K28_RS17535 Protein ID WP_000767822.1
Coordinates 3642781..3643035 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K28_RS17530 Protein ID WP_001259255.1
Coordinates 3642533..3642784 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K28_RS17505 (3637711) 3637711..3638778 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6K28_RS17510 (3638778) 3638778..3639848 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
I6K28_RS17515 (3639845) 3639845..3641149 - 1305 WP_001617113.1 histidinol dehydrogenase -
I6K28_RS17520 (3641155) 3641155..3642054 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K28_RS17525 (3642200) 3642200..3642250 - 51 WP_001364200.1 his operon leader peptide -
I6K28_RS17530 (3642533) 3642533..3642784 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K28_RS17535 (3642781) 3642781..3643035 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K28_RS17540 (3643118) 3643118..3643942 + 825 WP_000754733.1 SDR family oxidoreductase -
I6K28_RS17545 (3643988) 3643988..3644917 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
I6K28_RS17550 (3645132) 3645132..3645194 + 63 WP_010723108.1 membrane protein YoeI -
I6K28_RS17555 (3645184) 3645184..3646542 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T193210 WP_000767822.1 NZ_CP070115:3642781-3643035 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T193210 NZ_CP092779:1475913-1476263 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACAGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT193210 WP_001259255.1 NZ_CP070115:3642533-3642784 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT193210 NZ_CP092779:1475625-1475906 [Paenibacillus polymyxa]
TTGGCCAATTTGCAGAACACCAAAAGAATAATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGC
TATGGAGAATTCCAACCGCAGTGAATTTATCAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTC
GTGAAGCTATGCAGCGTGGATACATGGAAATGGCTAAAATTAACCTGACCATGGCATCTGAAGCCTTTCACGCGGAGGAA
GATGCAAACAGCACCTTGGGCCGTACAGTTAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References