Detailed information of TA system TA193205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1394402..1395004
• Replicon: chromosome
• Accession: NZ_CP070115
• Organism: Escherichia coli strain FDAARGOS_1298

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K28_RS06730
• Protein ID: WP_000897302.1
• Coordinates: 1394693..1395004 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K28_RS06725
• Protein ID: WP_000356397.1
• Coordinates: 1394402..1394692 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K28_RS06700 (1390475)	1390475..1391377	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K28_RS06705 (1391374)	1391374..1392009	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K28_RS06710 (1392006)	1392006..1392935	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K28_RS06715 (1393151)	1393151..1393369	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K28_RS06720 (1393765)	1393765..1394043	-	279	WP_001296612.1	hypothetical protein	-
I6K28_RS06725 (1394402)	1394402..1394692	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K28_RS06730 (1394693)	1394693..1395004	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K28_RS06735 (1395233)	1395233..1396141	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6K28_RS06740 (1396205)	1396205..1397146	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K28_RS06745 (1397191)	1397191..1397628	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K28_RS06750 (1397625)	1397625..1398497	-	873	WP_000920754.1	virulence factor BrkB family protein	-
I6K28_RS06755 (1398491)	1398491..1399090	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T193205 WP_000897302.1 NZ_CP070115:c1395004-1394693 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193205 NZ_CP092767:17676-18092 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT193205 WP_000356397.1 NZ_CP070115:c1394692-1394402 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193205 NZ_CP092767:17449-17679 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1P416