Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 182454..183133 Replicon chromosome
Accession NZ_CP070115
Organism Escherichia coli strain FDAARGOS_1298

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag I6K28_RS00960 Protein ID WP_000057524.1
Coordinates 182831..183133 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K28_RS00955 Protein ID WP_000806442.1
Coordinates 182454..182795 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K28_RS00945 (178698) 178698..179630 - 933 WP_000883052.1 glutaminase A -
I6K28_RS00950 (179892) 179892..182396 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
I6K28_RS00955 (182454) 182454..182795 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K28_RS00960 (182831) 182831..183133 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K28_RS00965 (183266) 183266..184060 + 795 WP_000365177.1 TraB/GumN family protein -
I6K28_RS00970 (184264) 184264..184743 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K28_RS00975 (184767) 184767..185567 + 801 WP_000439796.1 hypothetical protein -
I6K28_RS00980 (185564) 185564..186067 + 504 WP_000667000.1 hypothetical protein -
I6K28_RS00985 (186105) 186105..187757 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 175143..186067 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T193198 WP_000057524.1 NZ_CP070115:c183133-182831 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T193198 NZ_CP092765:c2681175-2679853 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT193198 WP_000806442.1 NZ_CP070115:c182795-182454 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT193198 NZ_CP092765:c2681441-2681175 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References