Detailed information of TA system TA193187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3266457..3267059
• Replicon: chromosome
• Accession: NZ_CP070111
• Organism: Escherichia coli strain FDAARGOS_1301

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K31_RS15685
• Protein ID: WP_000897302.1
• Coordinates: 3266748..3267059 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K31_RS15680
• Protein ID: WP_000356397.1
• Coordinates: 3266457..3266747 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K31_RS15655 (3262530)	3262530..3263432	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K31_RS15660 (3263429)	3263429..3264064	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K31_RS15665 (3264061)	3264061..3264990	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K31_RS15670 (3265206)	3265206..3265424	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K31_RS15675 (3265820)	3265820..3266098	-	279	WP_001296612.1	hypothetical protein	-
I6K31_RS15680 (3266457)	3266457..3266747	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K31_RS15685 (3266748)	3266748..3267059	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K31_RS15690 (3267288)	3267288..3268196	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6K31_RS15695 (3268260)	3268260..3269201	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K31_RS15700 (3269246)	3269246..3269683	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K31_RS15705 (3269680)	3269680..3270552	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K31_RS15710 (3270546)	3270546..3271145	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T193187 WP_000897302.1 NZ_CP070111:c3267059-3266748 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T193187 NZ_CP092759:4059411-4059629 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT193187 WP_000356397.1 NZ_CP070111:c3266747-3266457 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT193187 NZ_CP092759:4059010-4059384 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1P416