Detailed information of TA system TA193181

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 2643227..2643639
• Replicon: chromosome
• Accession: NZ_CP070111
• Organism: Escherichia coli strain FDAARGOS_1301

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A8E0IWC4
• Locus tag: I6K31_RS12730
• Protein ID: WP_001513534.1
• Coordinates: 2643298..2643639 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	2643227..2643303 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K31_RS12720 (2639839)	2639839..2641308	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
I6K31_RS12725 (2641308)	2641308..2643077	+	1770	WP_001513535.1	restriction endonuclease subunit S	-
- (2643227)	2643227..2643303	-	77	NuclAT_6	-	Antitoxin
- (2643227)	2643227..2643303	-	77	NuclAT_6	-	Antitoxin
- (2643227)	2643227..2643303	-	77	NuclAT_6	-	Antitoxin
- (2643227)	2643227..2643303	-	77	NuclAT_6	-	Antitoxin
- (2643227)	2643227..2643303	-	77	NuclAT_7	-	Antitoxin
- (2643227)	2643227..2643303	-	77	NuclAT_7	-	Antitoxin
- (2643227)	2643227..2643303	-	77	NuclAT_7	-	Antitoxin
- (2643227)	2643227..2643303	-	77	NuclAT_7	-	Antitoxin
I6K31_RS12730 (2643298)	2643298..2643639	+	342	WP_001513534.1	endoribonuclease SymE	Toxin
I6K31_RS25440 (2643686)	2643686..2644849	-	1164	WP_224153787.1	DUF1524 domain-containing protein	-
I6K31_RS25445 (2644903)	2644903..2645779	-	877	Protein_2517	DUF262 domain-containing protein	-
I6K31_RS12745 (2646185)	2646185..2647105	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
I6K31_RS12750 (2647290)	2647290..2648570	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	2627615..2643639	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12247.03 Da
• Isoelectric Point: 7.8545

>T193181 WP_001513534.1 NZ_CP070111:2643298-2643639 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASHYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T193181 NZ_CP092759:327958-328326 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT193181 NZ_CP070111:c2643303-2643227 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin