Detailed information of TA system TA193178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2293615..2294309
• Replicon: chromosome
• Accession: NZ_CP070111
• Organism: Escherichia coli strain FDAARGOS_1301

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K31_RS11090
• Protein ID: WP_001263500.1
• Coordinates: 2293615..2294013 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K31_RS11095
• Protein ID: WP_000554758.1
• Coordinates: 2294016..2294309 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K31_RS11065 (2288980)	2288980..2289438	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
I6K31_RS11070 (2289699)	2289699..2291156	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K31_RS11075 (2291213)	2291213..2291827	-	615	WP_000602123.1	peptide chain release factor H	-
I6K31_RS11080 (2291824)	2291824..2292963	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K31_RS11085 (2293153)	2293153..2293605	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K31_RS11090 (2293615)	2293615..2294013	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K31_RS11095 (2294016)	2294016..2294309	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K31_RS11100 (2294361)	2294361..2295416	-	1056	WP_001226168.1	DNA polymerase IV	-
I6K31_RS11105 (2295487)	2295487..2296272	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K31_RS11110 (2296244)	2296244..2297956	+	1713	Protein_2199	flagellar biosynthesis protein FlhA	-
I6K31_RS11115 (2298035)	2298035..2298193	+	159	WP_014639450.1	hypothetical protein	-
I6K31_RS11120 (2298276)	2298276..2298773	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2293615..2313968	20353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T193178 WP_001263500.1 NZ_CP070111:c2294013-2293615 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193178 NZ_CP092753:c5257008-5256625 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT193178 WP_000554758.1 NZ_CP070111:c2294309-2294016 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193178 NZ_CP092753:c5257250-5257008 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3