Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2045364..2046043 Replicon chromosome
Accession NZ_CP070111
Organism Escherichia coli strain FDAARGOS_1301

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag I6K31_RS09900 Protein ID WP_000057524.1
Coordinates 2045741..2046043 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K31_RS09895 Protein ID WP_000806442.1
Coordinates 2045364..2045705 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K31_RS09885 (2041608) 2041608..2042540 - 933 WP_000883052.1 glutaminase A -
I6K31_RS09890 (2042802) 2042802..2045306 + 2505 WP_016239198.1 copper-exporting P-type ATPase CopA -
I6K31_RS09895 (2045364) 2045364..2045705 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K31_RS09900 (2045741) 2045741..2046043 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K31_RS09905 (2046176) 2046176..2046970 + 795 WP_000365177.1 TraB/GumN family protein -
I6K31_RS09910 (2047174) 2047174..2047653 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K31_RS09915 (2047677) 2047677..2048477 + 801 WP_000439796.1 hypothetical protein -
I6K31_RS09920 (2048474) 2048474..2048977 + 504 WP_000667000.1 hypothetical protein -
I6K31_RS09925 (2049015) 2049015..2050667 - 1653 WP_001513633.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2038053..2048977 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T193176 WP_000057524.1 NZ_CP070111:c2046043-2045741 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T193176 NZ_CP092753:c4773850-4773566 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT193176 WP_000806442.1 NZ_CP070111:c2045705-2045364 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT193176 NZ_CP092753:c4774082-4773840 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References