Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1049201..1049763 Replicon chromosome
Accession NZ_CP070111
Organism Escherichia coli strain FDAARGOS_1301

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K31_RS04975 Protein ID WP_000605675.1
Coordinates 1049201..1049479 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K31_RS04980 Protein ID WP_000781370.1
Coordinates 1049479..1049763 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K31_RS04950 (1044788) 1044788..1045219 - 432 WP_000152310.1 peroxiredoxin OsmC -
I6K31_RS04955 (1045564) 1045564..1045779 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K31_RS04960 (1045881) 1045881..1046018 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K31_RS04965 (1046174) 1046174..1047871 + 1698 WP_000433462.1 malate dehydrogenase -
I6K31_RS04970 (1048005) 1048005..1049015 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K31_RS04975 (1049201) 1049201..1049479 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K31_RS04980 (1049479) 1049479..1049763 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K31_RS04985 (1049797) 1049797..1051143 - 1347 WP_000483767.1 IS4-like element IS4 family transposase -
I6K31_RS04990 (1051430) 1051430..1052083 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K31_RS04995 (1052076) 1052076..1052960 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1049797..1051125 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T193172 WP_000605675.1 NZ_CP070111:1049201-1049479 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T193172 NZ_CP092753:1953349-1953451 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT193172 WP_000781370.1 NZ_CP070111:1049479-1049763 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT193172 NZ_CP092753:c1953458-1953313 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References