Detailed information of TA system TA193160

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hokW-rdlD/-
• Location: 579097..579322
• Replicon: chromosome
• Accession: NZ_CP070111
• Organism: Escherichia coli strain FDAARGOS_1301

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: I6K31_RS02505
• Protein ID: WP_000813254.1
• Coordinates: 579167..579322 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	579097..579155 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K31_RS02460	574359..574511	-	153	WP_000379609.1	DUF1391 family protein	-
I6K31_RS02465	574783..575070	-	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6K31_RS02470	575070..575261	-	192	WP_000100899.1	hypothetical protein	-
I6K31_RS02475	575289..575690	-	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
I6K31_RS02480	575800..576072	+	273	WP_000887447.1	YdaS family helix-turn-helix protein	-
I6K31_RS02485	576056..576481	+	426	WP_000693918.1	toxin YdaT family protein	-
I6K31_RS02490	576553..577623	+	1071	WP_001262355.1	hypothetical protein	-
I6K31_RS02495	577664..578089	+	426	WP_001151152.1	DUF977 family protein	-
I6K31_RS02500	578264..578929	+	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
-	579097..579155	-	59	-	-	Antitoxin
I6K31_RS02505	579167..579322	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
I6K31_RS02510	579490..579768	+	279	WP_024193993.1	hypothetical protein	-
I6K31_RS02515	579770..580819	+	1050	WP_001265292.1	DUF968 domain-containing protein	-
I6K31_RS02520	580832..581206	+	375	WP_001217427.1	RusA family crossover junction endodeoxyribonuclease	-
I6K31_RS02525	581203..582024	+	822	WP_001235237.1	antitermination protein	-
I6K31_RS02530	582270..582983	+	714	WP_000342820.1	hypothetical protein	-
I6K31_RS02555	583708..583923	+	216	WP_000839572.1	class II holin family protein	-
I6K31_RS02560	583928..584242	+	315	WP_000193273.1	YdfR family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtP / ybtQ / ybtX / ybtS	559145..606437	47292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T193160 WP_000813254.1 NZ_CP070111:579167-579322 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T193160 NZ_CP092743:c1003518-1003120 [Leptospira interrogans]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGCGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAATAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT193160 NZ_CP070111:c579155-579097 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin