Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3398239..3398836 Replicon chromosome
Accession NZ_CP070105
Organism Klebsiella pneumoniae strain FDAARGOS_1314

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag I6K44_RS16520 Protein ID WP_004142563.1
Coordinates 3398239..3398556 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag I6K44_RS16525 Protein ID WP_004142561.1
Coordinates 3398549..3398836 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K44_RS16505 3393939..3395366 - 1428 WP_040236578.1 MFS transporter -
I6K44_RS16510 3395475..3396341 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
I6K44_RS16515 3397011..3398054 + 1044 WP_040236579.1 DUF2157 domain-containing protein -
I6K44_RS16520 3398239..3398556 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K44_RS16525 3398549..3398836 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
I6K44_RS16530 3399101..3399754 - 654 WP_004176973.1 oxygen-insensitive NAD(P)H nitroreductase -
I6K44_RS16535 3399874..3400242 - 369 WP_040236580.1 MmcQ/YjbR family DNA-binding protein -
I6K44_RS16540 3400242..3400823 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
I6K44_RS16545 3401003..3402118 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
I6K44_RS16550 3402149..3402490 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
I6K44_RS16555 3402508..3402756 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T193154 WP_004142563.1 NZ_CP070105:3398239-3398556 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T193154 NZ_CP092738:62615-62863 [Leptospira interrogans]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT193154 WP_004142561.1 NZ_CP070105:3398549-3398836 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT193154 NZ_CP092738:62857-63180 [Leptospira interrogans]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGAGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCAT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTTGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References