Detailed information of TA system TA193115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2470738..2471432
• Replicon: chromosome
• Accession: NZ_CP070103
• Organism: Escherichia coli strain FDAARGOS_1277

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: I6K07_RS11640
• Protein ID: WP_001263493.1
• Coordinates: 2471034..2471432 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: I6K07_RS11635
• Protein ID: WP_000554757.1
• Coordinates: 2470738..2471031 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K07_RS11615 (2466378)	2466378..2466875	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
I6K07_RS11620 (2467091)	2467091..2468803	-	1713	Protein_2271	flagellar biosynthesis protein FlhA	-
I6K07_RS11625 (2468775)	2468775..2469560	+	786	WP_000207549.1	putative lateral flagellar export/assembly protein LafU	-
I6K07_RS11630 (2469631)	2469631..2470686	+	1056	WP_001226164.1	DNA polymerase IV	-
I6K07_RS11635 (2470738)	2470738..2471031	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K07_RS11640 (2471034)	2471034..2471432	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K07_RS11645 (2471442)	2471442..2471894	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
I6K07_RS11650 (2472140)	2472140..2472343	+	204	Protein_2277	RtcB family protein	-
I6K07_RS11655 (2472342)	2472342..2472863	+	522	Protein_2278	peptide chain release factor H	-
I6K07_RS11660 (2472920)	2472920..2474377	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
I6K07_RS11665 (2474638)	2474638..2475096	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (2475692)	2475692..2475772	+	81	NuclAT_11	-	-
- (2475692)	2475692..2475772	+	81	NuclAT_11	-	-
- (2475692)	2475692..2475772	+	81	NuclAT_11	-	-
- (2475692)	2475692..2475772	+	81	NuclAT_11	-	-
I6K07_RS11670 (2475188)	2475188..2476432	+	1245	WP_085004531.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T193115 WP_001263493.1 NZ_CP070103:2471034-2471432 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T193115 NZ_CP092702:4448639-4448989 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT193115 WP_000554757.1 NZ_CP070103:2470738-2471031 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT193115 NZ_CP092702:4448394-4448645 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6