Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2464702..2465243 Replicon chromosome
Accession NZ_CP070103
Organism Escherichia coli strain FDAARGOS_1277

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag I6K07_RS11600 Protein ID WP_000615979.1
Coordinates 2464702..2464980 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag I6K07_RS11605 Protein ID WP_000729704.1
Coordinates 2464983..2465243 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K07_RS11585 (2462284) 2462284..2462862 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
I6K07_RS11590 (2463068) 2463068..2463835 + 768 WP_000333379.1 class II glutamine amidotransferase -
I6K07_RS11595 (2463806) 2463806..2464546 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
I6K07_RS11600 (2464702) 2464702..2464980 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
I6K07_RS11605 (2464983) 2464983..2465243 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
I6K07_RS11610 (2465429) 2465429..2466202 + 774 WP_001353765.1 C40 family peptidase -
I6K07_RS11615 (2466378) 2466378..2466875 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
I6K07_RS11620 (2467091) 2467091..2468803 - 1713 Protein_2271 flagellar biosynthesis protein FlhA -
I6K07_RS11625 (2468775) 2468775..2469560 + 786 WP_000207549.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T193114 WP_000615979.1 NZ_CP070103:c2464980-2464702 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T193114 NZ_CP092702:4352855-4353028 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT193114 WP_000729704.1 NZ_CP070103:c2465243-2464983 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT193114 NZ_CP092702:4352531-4352827 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References