193109

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 787734..788461 Replicon chromosome
Accession NZ_CP070103
Organism Escherichia coli strain FDAARGOS_1277

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag I6K07_RS03735 Protein ID WP_000550189.1
Coordinates 788147..788461 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6K07_RS03730 Protein ID WP_000560255.1
Coordinates 787734..788150 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K07_RS03720 (782994) 782994..785345 + 2352 WP_000695495.1 alpha-glucosidase -
I6K07_RS03725 (785671) 785671..787689 + 2019 WP_000121450.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
I6K07_RS03730 (787734) 787734..788150 - 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
I6K07_RS03735 (788147) 788147..788461 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
I6K07_RS03740 (788746) 788746..789882 - 1137 WP_000018690.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
I6K07_RS03745 (789967) 789967..790470 + 504 WP_001295542.1 M48 family metallopeptidase -
I6K07_RS03750 (790547) 790547..791230 + 684 WP_001183041.1 vancomycin high temperature exclusion protein -
I6K07_RS03755 (791309) 791309..792295 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T193109 WP_000550189.1 NZ_CP070103:c788461-788147 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T193109 NZ_CP092702:c3040802-3040395 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT193109 WP_000560255.1 NZ_CP070103:c788150-787734 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT193109 NZ_CP092702:c3041049-3040783 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References