Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4441317..4441919 Replicon chromosome
Accession NZ_CP070098
Organism Escherichia coli strain FDAARGOS_1370

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag I6L00_RS21860 Protein ID WP_000897305.1
Coordinates 4441317..4441628 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6L00_RS21865 Protein ID WP_000356395.1
Coordinates 4441629..4441919 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L00_RS21845 (4437250) 4437250..4437708 + 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -
I6L00_RS25930 (4438197) 4438197..4439084 + 888 Protein_4174 hypothetical protein -
I6L00_RS25935 (4439097) 4439097..4440011 + 915 WP_077634137.1 transposase -
I6L00_RS21855 (4440180) 4440180..4441088 - 909 WP_001306651.1 alpha/beta hydrolase -
I6L00_RS21860 (4441317) 4441317..4441628 + 312 WP_000897305.1 hypothetical protein Toxin
I6L00_RS21865 (4441629) 4441629..4441919 + 291 WP_000356395.1 NadS family protein Antitoxin
I6L00_RS21870 (4442004) 4442004..4442216 + 213 WP_000197769.1 hypothetical protein -
I6L00_RS21875 (4442278) 4442278..4442556 + 279 WP_001306650.1 hypothetical protein -
I6L00_RS21880 (4442975) 4442975..4443193 + 219 WP_001295676.1 CopG family transcriptional regulator -
I6L00_RS21885 (4443412) 4443412..4443654 + 243 WP_021523315.1 hypothetical protein -
I6L00_RS21890 (4443836) 4443836..4444765 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
I6L00_RS21895 (4444762) 4444762..4445397 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
I6L00_RS21900 (4445394) 4445394..4446296 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4437250..4437708 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T193096 WP_000897305.1 NZ_CP070098:4441317-4441628 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T193096 NZ_CP092702:c1269810-1269703 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT193096 WP_000356395.1 NZ_CP070098:4441629-4441919 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT193096 NZ_CP092702:1269857-1269923 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References